Quorum Sensing and Spoilage Potential of Psychrotrophic Enterobacteriaceae Isolated from Milk

Figure 7

(a) A representative thin-layer chromatogram of HalI expression in E. coli XL1-Blue cultured in LB medium. The spots were detected with E. coli pSB403 reporter strain. Standards: N-(3-oxohexanoyl)-L-homoserine lactone (OHHL); N-(hexanoyl)-L-homoserine lactone (HHL); N-(octanoyl)-L-homoserine lactone (OHL); (068) H. alvei wild type; AHL extract diluted 50 times in ethyl acetate; (HalI) E. coli XL1-Blue harboring pQE-30Xa-halI. (b) A representative thin-layer chromatogram of HalI expression in E. coli XL1-Blue cultured in LB medium. Spots were detected with C. violaceum CV026 reporter strain. Standards: N-(3-oxohexanoyl)-L-homoserine lactone (OHHL); N-(hexanoyl)-L-homoserine lactone (HHL); N-(octanoyl)-L-homoserine lactone (OHL); (HalI) E. coli XL1-Blue harboring pQE-30Xa-halI.

(a)

(b)