Effect of Adenosine and Adenosine Receptor Antagonists on Retinal Müller Cell Inwardly Rectifying Potassium Channels under Exogenous Glutamate Stimulation

Figure 2

The effect of glutamate treatment (Glu, mmol/L) on cultured rat Müller cells as determined by real time-PCR. The cultures were treated with different concentrations of glutamate (0-20 mmol/L) when approaching 80-100% confluence. Application of increasing concentrations of glutamate caused a dose-dependent decrease in the mRNA expression levels of Kir4.1, Kir2.1, GLAST, and GS. The effects were most remarkable when glutamate concentration was increased to 10 mmol/L (n=3, all P<0.05). indicated P<0.05 between the two groups. # indicated P<0.05 between the blank control group and any of the glutamate treatment groups.

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