Effect of Adenosine and Adenosine Receptor Antagonists on Retinal Müller Cell Inwardly Rectifying Potassium Channels under Exogenous Glutamate Stimulation

Figure 4

The effect of adenosine (Ado, μmol/L) on responses to 10 mmol/L-glutamate in cultured retinal rat Müller cells as determined by real time-PCR. The cultures received combined treatment of 10 mmol/L-glutamate and different concentrations of adenosine. The mRNA expression levels of Kir4.1, Kir2.1, GLAST, and GS were significantly increased by the addition of 5 μmol/L adenosine (n=3, all P<0.05). Continuing increase in the concentration caused decreases in mRNA expression levels. indicated P<0.05 compared with the Ado-0 group. indicated P<0.01 compared with the Ado-0 group. indicated P<0.01 compared with the Ado-0 group. # indicated significant difference compared with the Ado-5 group.

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