A Comparative Study of Three Detection Techniques for Leifsonia xyli Subsp. xyli, the Causal Pathogen of Sugarcane Ratoon Stunting Disease

Figure 8

Effects of different concentrations of Bst DNA polymerase on the LAMP reaction. (a) Detection of Lxx-LAMP products based on color change; (b) agarose gel electropherograms of Lxx-LAMP products. Lane M: 15,000 + 2,000 bp molecular marker; Lanes 0 U to 8.0 U: LAMP reaction mixture containing 20 ng/μL Lxx-positive plasmid plus 0 U, 2.0 U, 4.0 U, 6.0 U, and 8.0 U Bst DNA polymerase, respectively.

(a)

(b)