AEG-1 Contributes to Metastasis in Hypoxia-Related Ovarian Cancer by Modulating the HIF-1alpha/NF-kappaB/VEGF Pathway

Figure 5

AEG-1, HIF-1α, NF-κB, and VEGF expression levels in wild type and AEG-1 knockdown SKOV3 cells cultured under hypoxic conditions. (a) After AEG-1 knockdown by lentiviral infection, AEG-1, HIF-1α, NF-κB, and VEGF protein amounts were significantly reduced (all ) as assessed by Western blotting. (b) After AEG-1 knockdown, AEG-1, HIF-1α, and VEGF gene expression levels were significantly reduced (all ) as determined by real-time PCR. Data are from three independent experiments.

(a)

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