An Investigation on the Therapeutic Effect of Thymosin 4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice

Figure 3

Comparison of Tβ4 mRNA levels in the tissues of diabetic and control mice. STZ-induced type 1 diabetic mice and age-matched control mice were sacrificed at 16 weeks after the final injection of STZ. Each organ was harvested and the total RNA was extracted using Trizol. The Tβ4 transcriptional level was measured using real time PCR as described in the Materials and Methods. (a) In the control mice, the spleen had the highest expression level of Tβ4 among the various organs in relative to liver. A similar pattern was observed in the diabetic mice. The data represent triplicate samples of each organ of the control mice. (b) The Tβ4 expression level of each organ of the control mice was compared with the diabetic mice. There was no significant difference in Tβ4 expression between the control and diabetic mice. The representative results are shown. NS: not significant.

(a)

(b)