An Investigation on the Therapeutic Effect of Thymosin 4 and Its Expression Levels in Streptozotocin-Induced Diabetic Mice

Figure 5

Effects of glucose concentration on the in vitro expression of Tβ4 in splenocytes. Immune cells from the spleen were isolated and treated with high-glucose concentrations for 24 hrs. Subsequently, the total RNA was extracted from the cells for real time PCR analysis (a) and the cell culture supernatant was obtained to determine the Tβ4 levels using ELISA (b). Three independent experiments were performed using triplicates of each sample for the real time PCR and ELISA. Similar results were obtained from each experiment, and representative results are shown. NS: not significant.

(a)

(b)