Synergistic Effects of Controlled-Released BMP-2 and VEGF from nHAC/PLGAs Scaffold on Osteogenesis

Figure 8

Expression of GFP protein in MC3T3-E1 cells treated with osterix shRNA unloaded ((a) 3rd day after transfection) or loaded ((b) 3rd day and (c) 24th day after transfection) lentivirus (1. Images in fluorescent state; 2. Images in ordinary state). (d) Osterix protein expression of MC3T3-E1 cells treated with osterix shRNA loaded or unloaded lentivirus at the 3rd day and 24th day after transfection. (e) Expression of col1 protein in MC3T3-E1 cells treated with osterix shRNA loaded or unloaded lentivirus at 24th day after transfection (con: control, Mock: unloaded lentivirus; shRNA: loaded lentivirus).