Research Article
URG11 Regulates Prostate Cancer Cell Proliferation, Migration, and Invasion
Figure 2
Genetic knockdown of URG11 suppresses the proliferation and growth of prostate cancer cells. (a) Cultured LNCaP cells were transfected with siRNA fragments of NC (control) or against URG11 for 48 h. Then cells were harvested and subjected to western blot using URG11 antibody. Representative images were shown in the up panel; the statistical data of relative expression level compared to normal culture were shown in the lower panel. To determine the impact of URG11 knockdown on cell proliferation, LNCaP cells were cultured up to 4 days and cells were harvested at indicted time points for each group. Cell lysate was subjected to MTS. (b) OD 450 values, (c) growth rate, and (d) inhibition rate in each group were shown. All experiments were performed in triplicate, and results are expressed as the mean ± SD. denotes p < 0.05 versus NC control group.
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