Research Article

Optimization of Fluorescent Labeling for In Vivo Nanoimaging of Sarcomeres in the Mouse Heart

Table 1

Summary of the SL values via expression of α-actinin-AcGFP at various viral loads. See Figure 1 for details. compared with the value at 2×1011 VP/mL (10 μL). compared with the value at 4×1011 VP/mL (10 μL). means compared with the value at 2×1012 VP/mL (10 μL). Significant differences were tested by the Steel-Dwass test.

Titer (VP/mL)Volume (μL)SL (μm)Number of myocytesNumber of animalsNumber of sarcomeres

2×1011103831626
4×10111043177
2×101210184697
4×10113083347