Role of luxS in Stress Tolerance and Adhesion Ability in Lactobacillus plantarum KLDS1.0391

Table 1

Primer sequences.

Primer name	Sequence (5′-3′)	Annealing temperature (°C)	Function
luxS-L-f	CCGCTCGAGCGGTTTATCCCACT	57	Amplification of luxS left-flanking gene
luxS-L-r	AGCTTTGTTTAAACATTGCCCGTTATT
luxS-R-f	GAGCTCGGTGTACGCAAAGTCGT	63	Amplification of luxS right-flanking gene
luxS-R-r	GGAAGATCTAATTCCATGTTCACCAGC
pNZ5319-L-f	GAGCAGAATGTCCGAGAC	55	Identification of double-digested products
pNZ5319-L-r	CGGCTAAAACGACCTTAA
pNZ5319-luxS-f	TGTTGCCGATTCCGCTAG	55	Identification of double-digested products
pNZ5319-luxS-r	ACCCCGTCAGCTTTAGG
luxSqs-f	GTGAAAACGGTGGTGAGGTC	60	Identification of luxS gene mutant
luxSqs-r	TCTTTATGTGCTTTGAGCAATA