Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice

Figure 4

Acyl ghrelin influences the effect of LPS or IL-4 on RAW264.7 cell polarization. The mRNAs were extracted from the RAW 264.7 pretreated with acyl ghrelin for 30 minutes then for 6 hours with LPS (10 ng/ml) (a) or IL-4 (10 ng/ml) (b). Real-time PCR was performed to evaluate the expression of macrophage specific markers Arg-1 and MCP-1. Data are means±SEM from 3 separate experiments. denotes P<0.05 compared with control group; ^# denotes P<0.05 compared with cells treated with LPS or IL-4 alone.

(a)

(b)