Validation of Housekeeping Genes as Reference for Reverse-Transcription-qPCR Analysis in Busulfan-Injured Microvascular Endothelial Cells

Figure 6

Expression levels of the robo4 gene. The normal and Busulfan-injured endothelial cells were used, using wyhaz, the best validated reference gene for normalization. Data are mean ±SEM, n =3; P 0.01; P 0.001 vs. 0h calculated from the 3 independent replicates.