Proteomic Analysis of Liver from Human Lipoprotein(a) Transgenic Mice Shows an Oxidative Stress and Lipid Export Response

Figure 5

Increased relative abundance of Park7 in Lp(a) mice. Western blots were used to validate the comparative proteomic analysis between the Lp(a) and wildtype mice for the Park7 protein. Pooled liver protein extracts (n = 4 livers per pool) were separated by SDS PAGE in multiple replicates (n = 7) and transferred onto nitrocellulose membrane. Membranes were probed with an anti-Park7 antibody using an anti-actin antibody as a loading control. Liver protein extracts were the same as those used for Figure 4 as well as fresh liver protein extracts from new mice of the same age, sex, and genotype (b). Representative blots showing two of the replicates for each pooled liver protein extract are shown. Park7 protein levels were normalized against actin and expressed as a ratio in densitometry units (DU). Data is represented as mean ± SEM for the pooled samples run in septuplicate. P<0.05, ⁎⁎⁎P<0.001, versus wildtype.

(a)

(b)