BioMed Research International

Research Article

Carapa guianensis Aublet (Andiroba) Seed Oil: Chemical Composition and Antileishmanial Activity of Limonoid-Rich Fractions

Table 1

Activity against the promastigotes and intracellular amastigotes of Leishmania amazonensis, cytotoxicity in BALB/c peritoneal macrophages, and selectivity index of oil and limonoid-rich fractions of Carapa guianensis seed oil.


Compounds	IC₅₀ (μg/mL) promastigote	CC₅₀ (μg/mL) peritoneal macrophage	IC₅₀ (μg/mL) intracellular amastigote	IS

C. guianensis seed oil	>500	>1000	–	–
LF1	>500	>1000	–	–
LF2	>500	489.4 ± 0.173	–	–
LF3	10.53 ± 0.050	78.55 ± 1.406	27.31 ± 0.091	2.87
LF4	25.30 ± 0.057	139.0 ± 1.523	78.42 ± 0.086	1.77
LF5	56.90 ± 0.043	607.70 ± 1.217	352.2 ± 0.145	1.72
LF6	>500	>1000	–	–
Amphotericin B	1.37±0.124	6.26±0.286	0.2 ± 0.180	31.3

IC₅₀: inhibitory concentration of 50% parasites. CC₅₀: cytotoxicity concentration of 50% cells. SI: selectivity index calculated from the ratio of CC₅₀ versus the IC₅₀ for intracellular amastigotes. LF: limonoid-rich fraction of C. guianensis seed oil. Data represent mean ± standard deviation of three experiments performed at least in triplicate.