miR-144-5p Enhances the Radiosensitivity of Non-Small-Cell Lung Cancer Cells via Targeting ATF2

Figure 4

miR-144-5p targeted ATF2 in lung cancer cells. (a) The sequence alignment of human miR-144-5p with the 3′UTR of ATF2 is shown. The luciferase reporter constructs hsa-ATF2-wt and hsa-ATF2-mut were made using the seed sequence of miR-144-5p matching the 3′UTR of ATF2 mRNA. HEK293 cells were transiently cotransfected with the indicated plasmids and agomir-144 or agomir-NC for 48 h, and then they were subjected to the luciferase assay. versus agomir-NC. (b, c) A549 and H450 cells were transfected with agomir-144-5p or agomir-NC. The ATF2 protein (b) and mRNA (c) levels were determined by immunoblotting and RT-PCR, respectively. (d) The relative ATF2 mRNA levels in normal lung tissues (NLT, ) or lung cancer (LC, ) tissues were assessed using quantitative RT-PCR. (e) Correlation between the expression levels of ATF2 and that of mature miR-144-5p in 6 NLT and 40 LC tissues. versus agomir-NC. versus NLT.

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