Enhancing Bioethanol Productivity Using Alkali-Pretreated Empty Palm Fruit Bunch Fiber Hydrolysate

Figure 3

Effect of enzyme cocktail concentration on saccharification of alkali-pretreated empty palm fruit bunch fiber (EPFBF). (a) Fermentable sugar generation depended on the amount of Celluclast 1.5L/Novozyme 188 enzyme cocktail. (b) Sugar productivities of the enzyme cocktail ratio with the hydrolysis time: enzymatic saccharification was carried out at pH 4.8 and 42°C for 72 h. Then, 0.5 (open circle), 1.0 (open square), 1.5 (closed square), 2.0 (open triangle), and 2.5 FPU (closed circle) ratios of Celluclast 1.5L (FPU)/Novozyme 188 (CBU) were loaded per g dried EPFBF for enzymatic saccharification. All experiments were performed in triplicate. The data are expressed as mean ± SEM, n = 3.

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