LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

Figure 1

Schematic presentation of ClaI DNA fragment of 4239 bp carrying complete information for providing resistance to EcoRI digestion. ClaI DNA fragment containing following ORFs: truncated gene for pentapeptide repeat containing protein (Δorf1), EcoRI-like endonuclease (lraIR), EcoRI-like methylase (lraIM), hypothetical protein (hyp1), site specific integrase (intSS), and partial gene for N(5)-(carboxyethyl) ornithine synthase (par ceo).