LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity

Figure 6

Determination of the cleavage site of LraI activity. (a) Digestion of pBluescipt SK+ vector by LraI 29 (1 and 4), LraI 31 (2 and 5), and LraI 42 (3 and 6) in buffer recommended for use with EcoRI (1, 2, and 3) and in 1x Tango Buffer (4, 5, and 6); 7: undigested pBluescipt SK+; digestion of pAZIL vector by LraI 29 (1 and 4), LraI 31 (2 and 5), and LraI 42 (3 and 6) in buffer recommended for use with EcoRI (1, 2, and 3) and in 1x Tango Buffer (4, 5, and 6); 7: undigested pAZIL vector; L: ladder (GeneRuler DNA Ladder Mix, Thermo Fisher Scientific).

(a)

(b)