|
| Strains or plasmids | Relevant characteristics | Source or reference |
|
| Lactococcus raffinolactis | |
| BGTRK10-1 | Natural isolate from autochthonous sweet kajmak | [17] |
| Escherichia coli | |
| DH5α | supE44 ΔlacU169 (80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [21] |
| HB101 | hsdS20 () supE44 recA13 ara-14 proA2 lacY1 rpsL20() xyl-5 mtl-1 galK2 lacY1 | [24] |
| ER2523 | fhuA2 [lon] ompT gal sulA11 R(mcr- 73::miniTn10- -TetS) 2 [dcm] R(zgb-210::Tn10- -TetS) endA1 Δ(mcrC-mrr)114::IS10 | New England Biolabs, Ltd. UK |
| ER2523/pAZIL-LraRM | Competent cells obtained by transformation of ER2523 cells with pAZIL-LraRM | This study |
| Plasmids | |
| pAZIL | 7109 bp, , shuttle cloning vector | [19] |
| pAZIL-LraRM | PCR fragments of LraI operon from BGTRK10-1 cloned into pAZIL vector predigested with SmaI | This study |
| pAZIL-LraI672pBS | DNA fragment of 672 bp obtained after digestion of pBluescipt SK+ with LraI activity cloned into pAZIL vector predigested with LraI | This study |
| pAZILcos | 8194 bp, , shuttle cosmid vector | [19] |
| pAZILcosLra | Cosmid selected from total XbaI cosmid library of BGTRK10-1 | This study |
| pBluescript SK+ | 2958 bp, , cloning vector | Stratagene |
| pBSLraCla | ClaI fragment of 4239 bp obtained from pAZILcosLra cloned into pBluescript SK+ vector | This study |
| pMAL-c5X | 5677 bp, pMB1 origin, lacI, malE, bla, Factor Xa cleavage site; | New England Biolabs, Ltd. UK |
| pMAL-cX5LraI-29 | PCR fragment of LraI restriction endonuclease from pAZIL-LraRM cloned into pMAL-c5X vector predigested with XmnI and HindIII restriction enzymes | This study |
| pMAL-cX5LraI-31 | PCR fragment of LraI restriction endonuclease from pAZIL-LraRM cloned into pMAL-c5X vector predigested with XmnI and HindIII restriction enzymes | This study |
| pMAL-cX5LraI-42 | PCR fragment of LraI restriction endonuclease from pAZIL-LraRM cloned into pMAL-c5X vector predigested with XmnI and HindIII restriction enzymes | This study |
|
