Inflammatory Cellular Response to Mechanical Ventilation in Elastase-Induced Experimental Emphysema: Role of Preexisting Alveolar Macrophages Infiltration

Figure 5

Activation markers of alveolar macrophages (a) and pulmonary monocytes (b), analyzed by flow cytometry on total lung cell suspensions of mice subjected to spontaneous (SV) or mechanical (MV) ventilation 14 days after instillation of saline (Saline) or elastase (Ela), expressed as mean fluorescent intensity (MFI). (a) CD11b and Gr1 expression, and autofluorescence of alveolar macrophages. (b) CD11b, Gr1, and CD62L expression on pulmonary monocytes. Values are expressed as median ± interquartile range. n = 3-5 animals/group. ^£ denotes Benjamini-Hochberg corrected p value <0.10 (marginally significant) for the following Mann-Whitney pairwise comparisons (following Kruskal-Wallis test): Saline SV versus Saline MV, Saline SV versus Ela SV, Ela SV versus Ela MV, Saline MV versus Ela MV. Definition of abbreviations: Ela: elastase; SV: spontaneous ventilation; MV: mechanical ventilation.

(a) Alveolar macrophages

(b) Pulmonary monocytes