Development of Novel Single-Chain Antibodies against the Hydrophobic HPV-16 E5 Protein

Figure 1

W12 cells were subjected to multiplex PCR for the E2 and E6 genes. (a) Specific fragments of the E2 (352 bp-upper band) and E6 genes (208 bp-lower band) were successfully coamplified and separated on a 1.5% agarose gel. Lanes: 1, DNA marker; 2, DNA amplification using only E6 primers; 3, DNA amplification using E2 and E6 primers. Numbers on the left side represent bps. (b) qPCR analysis of both E2 and E6. Ct, cycle threshold.

(a)

(b)