Neuronal Signal Transduction-Involved Genes in Pig Hypothalamus Affect Feed Efficiency as Revealed by Transcriptome Analysis

Figure 2

Differential expression analysis in the high- and low-FE pigs. (a) Differential expression patterns of annotated genes and lincRNAs on the two porcine genome versions scaled by log₂FC (|log₂FC| > 1, FDR < 0.05). (b) Comparison of the Venn diagram of DE genes between two genome versions. (c) Plot of the DE-annotated genes and lincRNAs with the threshold of |log₂FC| > 1, FDR < 0.05. The x- and y-axes represent log₂FC and log₁₀(p value), respectively. (d) The RNA-seq and qPCR verification of DE genes and lincRNAs in the hypothalamic tissue of high- and low-FE pigs (n = 12, 6 high-FE pigs versus 6 low-FE pigs); represents p < 0.05 and p < 0.01. (e) Correlation analysis of RNA-seq data and qPCR results with log₂FC value. log₂FC, log₂Fold Change (FE high/low).

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