Targeting YOD1 by RNA Interference Inhibits Proliferation and Migration of Human Oral Keratinocytes through Transforming Growth Factor-3 Signaling Pathway

Figure 4

YOD1 played important roles by affecting the activation of TGF-β3/Smad signaling pathway. (a) qRT-PCR showed that the mRNA expression of TGF-β3 in the HOK cells transfected with YOD1-specific siRNA was decreased compared with the control groups. Data are shown as mean ± SD of three replicate experiments from each group. versus the negative control. (b) Protein levels of YOD1, TGF-β3, TβRI, TβRII, total Smad2/3, p-Smad2/3 and Smad4 were determined by western blot analysis. Representative western blots are shown. Densitometric analysis of western blots was used to quantify protein expression. Quantitative densitometry evaluation of (c) YOD1, (d) TGF-β3, (e) TβRI, (f) TβRII, (g) Smad2, (h) Smad3, (i) p-Smad2, (j) p-Smad3, and (k) Smad4 was shown. Results are presented as fold-change compared to control. Blots were normalized with tubulin for total extracts. Data are shown as mean ± SD of three replicate experiments from each group. versus the negative control.

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