Anthocyanins Function as Anti-Inflammatory Agents in a Drosophila Model for Adipose Tissue Macrophage Infiltration

Figure 3

Anthocyanins-rich diet reduces the infiltration of hemocytes in the FBs and the phosphorylation of JNK/SAPK. (a) Scheme of the different diets NF (Normal Food) or enriched in FL (flavonoids) or ACN (anthocyanins). (b) % of hemocytes in the cells of the FBs from animals at the indicated genotypes and fed with the indicated diets at 5 or 12 days AEL. (c) Confocal images showing hemocytes expressing Hml-RFP (red) and nuclei stained with Hoechst (blue) from animals at 12 days AEL, upon feeding with NF, FL, or ACN enriched diets. (d) Western blot from lysates of FBs showing the level of phosphorylation of JNK/SAPK p46 kinase, in P0206-Gal4 (control) and P0206-Gal4; UAS-Ni animals fed in FL or ACN enriched diets. FBs were taken at 5 or 12 days AEL. Actin was used as control loading. (e) Model of JNK signaling and potential action of anthocianins. Error bars represent SEM (standard error of the mean) of three independent experiments. and .

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