Differential Modulation of Transcription Factors and Cytoskeletal Proteins in Prostate Carcinoma Cells by a Bacterial Lactone

Figure 3

3D Cell viability assay. Different cells (10⁴ cells/well) were grown in matrigel precoated chamber slide wells. The cells were grown for 7 days with change in cell medium once every two days. After one week, C12-HSL (75 μmol/L) was added consecutively for 5 days with change in cell media and maintained at 37°C for the duration of the study. After C12-HSL treatment, the cells were washed with 1x PBS and were imaged in an Olympus CK40 phase contrast inverted microscope to image the morphology of the cells. Inset. As a separate experiment, Calcein AM dye (Ex/Em = 494/520 nm) (Biotium, Hayward, CA) was added to the cells after C12-HSL treatment and incubated for 2 h at 37°C to test the cell viability and cell membrane integrity. The cells were imaged using an Olympus CK40 fluorescent microscope. The fluorescence intensity measurements were created with ImageJ (NIH). The fluorescent intensities are depicted as CTCF values in the graph. ns, not significant; and .