Differential Modulation of Transcription Factors and Cytoskeletal Proteins in Prostate Carcinoma Cells by a Bacterial Lactone

Figure 6

C12-HSL modulation of different proteins. (a) Western blot analysis for STAT3 (~80 kDa), pSTAT3 (Tyr 705; ~80 kDa), STAT1 (~81 kDa), pSTAT1 (Tyr 701) (~81 kDa), CDKN1A (~19 kDa), and pCDKN1A (Thr 145; ~19 kDa) in RWPE-1, PC3, LNCaP, and DU145 cells with or without treatment with C12-HSL (75 μmol/L) for 24 h. (b) Western blot analysis for Cofilin, IQGAP1 (~195 kDa), RhoC (~23 kDa), and vinculin (~135 kDa) in RWPE-1, PC3, LNCaP, and DU145 cells with or without treatment with C12-HSL (75 μmol/L) for 24 h. GAPDH (~38 kDa) was used as a control protein. (c) Graphs depicting the fold changes in different proteins. The intensity of the protein bands was measured using ImageJ program. Based on the raw data, the WT cells were assigned an arbitrary value of 1. Protein fold changes in C12-HSL treated cells were relative to WT cells. and .

(a)

(b)

(c)