Differential Modulation of Transcription Factors and Cytoskeletal Proteins in Prostate Carcinoma Cells by a Bacterial Lactone

Figure 7

Fluorescence microscopy analysis of actin in PC3 cells. WT and C12-HSL treated (50 μmol/L, 1 h at 37°C) PC3 cells were analyzed using Acti-stain 488 fluorescent phalloidin. WT PC3 (a and b) cells exhibited more organized actin filaments. However, C12-HSL treatment induced changes in the actin filaments and appeared more punctuated (c–f). Cell nuclei were stained with DAPI (b, d, f) or were not stained with DAPI for clarity (a, c, e). Images are of 60x magnification.

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