Human MLL-AF9 Overexpression Induces Aberrant Hematopoietic Expansion in Zebrafish

Figure 1

MLL-AF9 overexpression in zebrafish embryos and activation of MLL downstream genes. MLL-AF9 mRNA was injected into zebrafish at the 1–2-cell stage (around 5ng per embryo). Injected embryos and control embryos were harvested at 48hpf. (a) Western blotting of the N-terminus of the MLL protein in MLL-AF9-injected and control embryos. (b) Relative expression of MLL downstream genes analyzed by qRT-PCR. (c–e) WISH assays of hoxa9a, hoxb5a, and meis1 in MLL-AF9-injected and control embryos at 24hpf. (f, g) Morphology of the hematopoietic cells of control embryos and MLL-AF9-injected embryos at 48 hpf.

(a)

(b)

(c)

(d)

(e)

(f)

(g)