Effects of Astaxanthin on Miniature Pig Sperm Cryopreservation

Table 2

ROS evaluation of ethidium and DCF fluorescence in frozen-thawed boar sperm of control and astaxanthin (AXT).

Sperm treatment	Viable sperm with high (%)	MFI of ethidium/total sperm	MFI of ethidium/viable sperm	Viable sperm with high (%)	MFI of DCF/total sperm	MFI of DCF/viable sperm
Control	31.5 ± 1.	116.8 ± 20.4	135.0 ± 24.0	4.0 ± 0.8	72.2 ± 0.	87.7 ± 2.
AXT 10 μM	44.4 ± 1.	106.9 ± 22.8	114.0 ± 23.0	4.8 ± 0.9	83.0 ± 8.	95.7 ± 7.
AXT 50 μM	43.7 ± 1.	118.0 ± 6.6	126.6 ± 13.8	4.0 ± 0.8	73.8 ± 6.	87.8 ± 8.
AXT 100 μM	41.1 ± 0.	116.7 ± 10.5	121.2 ± 9.9	2.7 ± 0.9	65.6 ± 4.	76.0 ± 4.
AXT 500 μM	40.8 ± 0.	124.3 ± 16.1	130.7 ± 16.4	4.3 ± 0.3	78.0 ± 2.	95.1 ± 2.
value

a, b, and c indicate difference , , and , respectively.