Establishment of a Gene Detection System for Hotspot Mutations of Hearing Loss

Table 5

Recommended melting conditions on three qPCR platforms.

Platforms	Recommended instrument setting	Measured ramp rate (°C/s)	Total time (min)
LightCycler 96	5 readings/°C	0.05	30
LightCycler 480	5 acquisitions/°C	0.03	45
Bio-Rad CFX-96	Step (0.3°C)/hold (5 s)	0.01	60