EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Figure 1

EGF induces EMT in primary HLECs. (a) The primary HLECs were treated with different concentrations of EGF for 48 h. The expression of E-cadherin proteins in primary HLECs was determined by Western blot. (b) The primary HLECs were treated with 10 ng/mL of EGF for different time. The expression of E-cadherin proteins was measured by Western blot. (c–f) The expression of fibronectin mRNA and α-SMA mRNA was measured by qRT-PCR. (c, e) The primary HLECs were treated with different concentrations of EGF for 48 h. (d, f) The primary HLECs were treated with 10 ng/mL of EGF for different time. compared with group without EGF.

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