EGF-Mediated Overexpression of Myc Attenuates miR-26b by Recruiting HDAC3 to Induce Epithelial-Mesenchymal Transition of Lens Epithelial Cells

Figure 3

EGF induces the expression of Myc and EZH2 in primary HLECs. (a) Effects of EGFR siRNA on EGFR expression in primary HLECs. We achieved downregulation of EGFR as monitored by Western blot analysis. (b) After 48 h of EGF (10 ng/ml) treatment, MYC and EZH2 were indeed overexpressed in primary HLECs by Western blot analysis. Moreover, EGFR knockdown led to downregulation of Myc and EZH2 expression induced by EGF. ((c) and (d)) After 48 h of EGF (10 ng/ml) treatment, MYC mRNA (c) and EZH2 mRNA (d) were overexpressed as monitored by qRT-PCR. compared with normal.

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