Protective Role of Coxsackie-Adenovirus Receptor in the Pathogenesis of Inflammatory Bowel Diseases

Figure 1

CAR expression is inhibited by inflammatory factor TNF-α treatment in human colon cell lines. (a) qRT-PCR detection of CAR mRNA levels in human colon cell lines upon TNF-α treatment. Human colon mucosal epithelium cell line NCM460 and human colon cancer cell line SW480 were treated with control or TNF-α (10 ng/ml) for 24 hours before cells were harvested for qRT-PCR assay. (b) Western-blot detection of CAR protein level after CAR overexpression. NCM460 and SW480 were transfected with control vector or pEGFP-N1-CAR overexpression plasmid, 48 hours after transfection, cells were harvested for protein extraction, and CAR protein level was determined using antibody that specifically recognizes CAR. (c) Quantitative analysis of CAR protein levels (calibrated with β-actin levels of each sample) in (b). , , and , and data represent mean ± s.e.m.

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