Upregulated TSG-6 Expression in ADSCs Inhibits the BV2 Microglia-Mediated Inflammatory Response

Figure 11

miR-214-5p negatively regulates the immunosuppressive capacity of ADSCs. Results are representative of three independent experiments using three different ADSCs cultures. There are six treatment groups in total, group 1 (BV2 microglia), group 2 (BV2 microglia+LPS), group 3 (BV2 microglia+LPS+miR214 5p-mimic ADSCs), group 4 (BV2 microglia+LPS+miR214 5p-mimic control ADSCs), group 5 (BV2 microglia+LPS+miR214 5p-inhibitor ADSCs), and group 6 (BV2 microglia+LPS+miR214 5p-inhibitor control ADSCs). IL-1β, IL-6, iNOS, and TNFα were decreased in ADSCs transfected with miR-214-5p inhibitor as compared to the inhibitor control; increased in ADSCs transfected with miR-214-5p mimic as compared to the mimic control. Comparison with group1, P<0.05; with group2, #P<0.05; with group 3, ∘P<0.05; and with group5, P<0.05.