Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture

Figure 4

The percentage of permeabilized cells (a) and the propidium iodide fluorescence measured right after the electroporation with 10 μM concentration of propidium iodide on three cell lines of PDA: primary cell culture of PDA metastatic cells from lung (b); EPP85-181P (sensitive to daunorubicin (c)) and EPP85-181RDB (resistant to daunorubicin (d)). The colors are assigned to the electric field strength: grey, 0 V/cm; green, 400 V/cm; blue, 600 V/cm; navy blue, 800 V/cm; violet, 1000 V/cm; pink, 1200 V/cm; orange, 1400 V/cm; red, 1600 V/cm; statistically significant differences between cells exposed to EP and nontreated control ().

(a)

(b) PDA primary cell culture

(c) EPP85-181P

(d) EPP85-181RDB