miR-548b-3p Regulates Proliferation, Apoptosis, and Mitochondrial Function by Targeting CIP2A in Hepatocellular Carcinoma

Figure 2

miR-548b-3p inhibits HCC cell proliferation and cell cycle transition. (a) Expression of miR-548b-3p in normal liver cell line LO2(HL-7702) and 3 HCC cell lines (SK-Hep-1, Bel7402, and Huh7). miR-548b-3p mimic upregulated its expression in Huh7 cell line and miR-548b-3p inhibitor suppressed its expression in SK-Hep-1 cell line. (b) MTT assay demonstrated that miR-548b-3p mimic suppressed Huh7 cell proliferation while miR-548b-3p inhibitor accelerated SK-Hep-1 cell proliferation. (c) Colony formation assay demonstrated that miR-548b-3p mimic reduced colony numbers in Huh7 cells while miR-548b-3p inhibitor upregulated colony numbers in SK-Hep-1 cells. (d) Cell cycle analysis showed that miR-548b-3p mimic upregulated G1 phase percentage and downregulated S phase percentage. In contrast, miR-548b-3p inhibitor downregulated G1 phase percentage and upregulated S phase percentage.

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