Microcrystalline Cellulose for Delivery of Recombinant Protein-Based Antigen against Erysipelas in Mice

Figure 2

SDS-PAGE and Western blot analyses of the CBD-SpaA-H6 expressed in E. coli BL21(DE3)/pKPM-CBD-SpaA-H6: (a) SDS-PAGE; (b) Western blot with anti-histidine antibody; (c) anti-serum derived from mouse inoculated with whole E. rhusiopathiae. Lane M: standard molecular weight marker, T: total cell lysates, and S: soluble fraction proteins. The arrows indicate the expressed CBD-SpaA.

(a)

(b)

(c)