Research Article
Improvement Activity of 1-Deoxynojirimycin in the Growth of Dairy Goat Primary Mammary Epithelial Cell through Upregulating LEF-1 Expression
Figure 2
Improvement activity of DNJ in primary mammary epithelial cell growth through upregulating LEF-1 expression. (a) A similar effect of DNJ with LEF-1 overexpression on the primary mammary epithelial cell growth. Dairy goat primary mammary epithelial cells were added to a 6-well plate at a density of 3 × 105/cm2, which were treated with 6 μM DNJ, adenovirus-mediated LEF-1 overexpression vector (pAd-LEF-1), lentivirus-mediated LEF-1 knockdown vector (Lenti-LEF-1), Lenti-LEF-1 + 6 μM DNJ, or pAd-LEF-1 + 6 μM DNJ for 72 h, respectively, and observed using a microscope (100x). (b) Detection of LEF-1 mRNA levels in each group of (a) through real-time qPCR. (c) Relative growth of the primary mammary epithelial cell in each group of (a). Data were expressed as mean ± SEM, compared with control (DMSO); indicated ; indicated .
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