Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana

Table 4

Fatty acid methyl ester composition from Soxhlet (8 hours using n-hexane) and SWE extraction (Run 15 at 215°C, 15 mins, and 75 g algae/L).

Fatty Acid Methyl Ester (FAME)	Structure	Fatty Acid Composition (wt of total FAME)
		Soxhlet	SWE (Run 15)
Caprylic	C8:0	0.069	0
Capric	C10:0	0.062	0
Undecanoic	C11:0	0.848	0.737
Lauric	C12:0	0.334	0.990
Tridecanoic	C13:0	0.215	2.020
Myristic	C14:0	2.578	5.491
Pentadecanoic	C15:0	0.209	0.378
Cis-10-Pentadecenoic	C15:1	0	0.284
Palmitic	C16:0	18.031	29.269
Palmitoleic	C16:1	22.849	35.581
Margaric	C17:0	0.205	0.312
Cis-10-Heptadecenoic	C17:1	0.164	0
Stearic	C18:0	0.436	0.481
Oleic	C18:1n9c	3.566	3.386
Linoleic	C18:2n6c	2.315	1.914
-Linolenic	C18:3n6	0.337	1.072
-Linolenic	C18:3n3	4.006	0
Cis-11,14-Eicosadienoic	C20:2	0.996	0
Cis-11,14,17-Eicosatrienoic	C20:3n3	0.763	1.037
Cis-8,11,14-Eicosatrienoic	C20:3n6	3.083	2.434
Arachidonic	C20:4n6	0.514	0
Eicosapentaenoic (EPA)	C20:5n3	28.103	14.135
Erucic	C22:1n9	6.393	0.478
Docosadienoic	C22:2	2.259	0
Tricosanoic	C23:0	1.664	0
Saturated Fatty Acid (SFA) (wt of total FAME)		25.647	39.678
Monounsaturated Fatty Acid (MUFA) (wt of total FAME)		32.973	39.730
Polyunsaturated Fatty Acid (PUFA) (wt of total FAME)		42.376	20.592
Omega-3 (wt of total FAME)		32.873	15.172
Omega-6 (wt of total FAME)		6.249	5.420
Lipid Yield (wt of total biomass)		10.200	17.860