Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana

Table 5

ANOVA of all factors and summary statistics of lipid yield.

Response variables	Source	Sum of squares	DF	Mean square	F-value	p value
Y1	Model	202.934	7	28.991	17.067	0.0002
	Linear
	A	67.555	1	67.555	39.770	0.0001
	B	0.126	1	0.126	0.074	0.7915
	C	47.109	1	47.109	27.733	0.0005
	Quadratic
	A^2	42.431	1	42.431	24.979	0.0007
	B^2	50.572	1	50.572	29.772	0.0004
	C^2	17.097	1	17.097	10.065	0.0113
	Interaction
	BC	15.015	1	15.015	8.840	0.0156
	Residual	15.288	9	1.699
	Lack of Fit	12.770	7	1.824	1.449	0.4673
	Pure Error	2.517	2	1.259
	Corrected Total	218.222	16
	Summary Statistics
	R^2	0.9299
	Adjusted R^2	0.8755
	Predicted R^2	0.7381
	Adequate Precision	12.1945

A: temperature (°C), B: extraction time (min), and C: biomass loading (g algae/L)