Optimization of Subcritical Water Extraction (SWE) of Lipid and Eicosapentaenoic Acid (EPA) from Nannochloropsis gaditana

Table 6

ANOVA of all factors and summary statistics of EPA composition.

Response variables	Source	Sum of squares	DF	Mean square	F-value	p value
	Model	0.136	3	0.0429	42.389	< 0.0001
	Linear
	A	0.084	1	0.084	78.358	< 0.0001
	C	0.005	1	0.005	4.703	0.0492
	Quadratic
	A^2	0.047	1	0.047	44.107	< 0.0001
	Residual	0.014	13	0.001
	Lack of Fit	0.013	11	0.001	1.747	0.4199
	Pure Error	0.001	2	0.001
	Corrected Total	0.150	16
	Summary Statistics
	R^2	0.9073
	Adjusted R^2	0.8859
	Predicted R^2	0.8526
	Adequate Precision	21.388

A: temperature (°C), B: extraction time (min), and C: biomass loading (g algae/L)