Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

<div>The schematic diagram and identification of pBRATm-rRSV-EGFP. (a) The recombinant full-length RSV antigenomic cDNA cloned from wide-type RSV Long strain with the inserted EGFP gene expression cassette in the position between G and F and flanked by T7 promoter and HDV ribozyme-T7 terminator in the 5′ and 3′ ends, respectively. T7 pro: T7 promoter; T7 ter: T7 terminator; Lead: Leader; Trail: Trailer; Ribozyme: HDV ribozyme. (b) Identification of pBRATm-RSV-EGFP by restriction endonuclease analysis. M, DNA Ladder DL15000; pBRATm-RSV-EGFP was digested with Asu II and Nhe I (1), Pme I and Xho I (2), Asu II and Sac I (3), EcoR V (4), Spe I (5), Afl II (6), or Mlu I (7).</div>

BioMed Research International

fig1

Figure 1

Figure 1: Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay 

Figure 1 | Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay 