Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

Figure 2

Recovery of rRSV-EGFP. (a) BHK-T7/9 cells cotransfected with pBRATm-RSV-EGFP and four helper plasmids (1) or cotransfected with only four helper plasmids (mock). (b) The suspensions from BHK-T7/9 cells cotransfected by pBRATm-RSV-EGFP and four helper plasmids (1), or only by four helper plasmids (mock) inoculated into HEp-2 cells for the blind passage. The fluorescence signals were observed around 120 h and about 72 h under the inverted fluorescence microscope following the posttransfection and the first blind passage, respectively.

(a)

(b)