Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

<div>Characterization of rRSV-EGFP. (a) Identification of the rescued rRSV-EGFP by RT-PCR. M, DNA Ladder DL2000; control of HEp-2 cells (1),<i> wt</i>RSV Long infected HEp-2 cells (2), or rRSV-EGFP infected HEp-2 cells (3). (b) The replication titers of rRSV-EGFP during serial blind passages from p1 to p9 by immunoplaque assay. (c) The growth kinetic of rRSV-EGFP. The growth curve for rRSV-EGFP was compared with those for<i> wt</i>RSV Long, rRSV-RFP, and rRSV-Luc. Each virus was harvested every other 24 h postinfection and titers were assayed by immunoplaque assay. (d) The replication capacity of the rRSV-EGFP in HEp-2, Vero, or 293T cells. Viruses were harvested at 48 h postinfection and titers were determined by assays of immunoplaque and RT-qPCR. 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BioMed Research International

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Figure 3

Figure 3: Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay 

Figure 3 | Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay 