Construction and Characterization of a Recombinant Human Respiratory Syncytial Virus Encoding Enhanced Green Fluorescence Protein for Antiviral Drug Screening Assay

Figure 4

Inhibitive activities of antiviral agents on the replication of rRSV-EGFP in HEp-2 cells determined and compared with rRSV-RFP, rRSV-Luc, and wtRSV Long. The fluorescence signals from rRSV-EGFP or rRSV-RFP were observed under invert fluorescence microscope (a-b), the titers from rRSV-EGFP, rRSV-RFP, rRSV-Luc, or wtRSV Long were analyzed by immunoplaque and RT-qPCR (c-d), and the intensities of fluorescence or luminescence from rRSV-EGFP, rRSV-RFP, or rRSV-Luc were detected by multifunctional microplate reader and expressed as relative fluorescence units (RFU) and relative light units (RLU), respectively (e–g), following treatments with 10 μmol/L ribavirin (1), 0.5 μmol/L compound P13 (2), 0.4 μmol/L mycophenolic acid (3), 1.5 μmol/L dequalinium chloride (4), or no antiviral agent (mock) for 48 h. And HEp-2 cells were used as control of background levels (-). Data were shown as mean ± SD. , .

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