Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane

Figure 3

Sensitivity tests for two sets of real-time quantitative PCR (qPCR) primers of MOR-F2/MOR-R2 and IM-F2/IM-R2 and a set of conventional PCR primers SCBV-F/SCBV-R using gel electrophoresis. (a) Serial dilutions (10⁸–10 copies/μL) of pMD18T-IM plasmid DNA with SCBV-F/SCBV-R primers. (b) Serial dilutions (10⁸–10 copies/μL) of pScBV20 plasmid DNA with SCBV-F/SCBV-R primers. (c) Serial dilutions (10⁸–10 copies/μL) of pMD18T-IM plasmid DNA with IM-F2/IM-R2 primers. (d) Serial dilutions (10⁸–10 copies/μL) of pScBV20 plasmid DNA with MOR-F2/MOR-R2 primers. M1, DNA Marker DL2,000; M2, 20 bp DNA Ladder Marker; NC, total DNA (100 ng/μL) of SCBV-negative sugarcane leaf; H₂O, blank control.

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