Development of Quantitative Real-Time PCR Assays for Rapid and Sensitive Detection of Two Badnavirus Species in Sugarcane

Table 3

Detection of sugarcane bacilliform viruses (SCBVs) in sugarcane leaf samples from Fujian and Yunnan provinces using real-time quantitative PCR (qPCR) and conventional PCR.

Location	No. of sugarcane varieties tested	No. of leaf samples tested	qPCR					Conventional PCR
			SCBIMV	Copies/µL	SCBMOV	Copies/µL	Coinfection
Fuzhou, Fujian	38	114	81 (71%)	5.7×10^1-1.7×10^4	66 (58%)	3.8×10^1-1.5×10^3	47 (41%)	46 (40%)
Boshang, Yunnan	7	8	0	0	3 (38%)	3.4×10^1-7.1×10^1	0	0
Dehong, Yunnan	13	13	0	0	2 (15%)	2.7×10^1-3.8×10^1	0	1 (8%)
Lingcang, Yunnan	35	41	7 (17%)	6.4×10^1-1.1×10^2	12 (29%)	1.8×10^1-3.0×10^2	4 (10%)	4 (10%)
Total	93	176	88 (50%)	0	83 (47%)	0	51 (29%)	51 (29%)