Research Article
Panax notoginseng Saponins Regulate Macrophage Polarization under Hyperglycemic Condition via NF-κB Signaling Pathway
Figure 4
PNS prompt arginase 1 expression by decreasing IκB-alpha phosphorylation in macrophages under hyperglycemic conditions. THP-1 macrophages were pretreated with 5uM/l BAY 11-7082 for 2 hours before they were stimulated with 15mMol/l glucose for 24 hours. In this experiment, cell culture time for PNS group is also 24 hours. Effects of 60ug/ml PNS and 5uM/l BAY 11-7082 on IκB-alpha phosphorylation (a, c) and arginase 1 expression (b, d) were determined by Western blot (n=3 per group). Results are expressed as the mean ± SE. indicates p<0.05 and indicates p<0.01.