Epidermal Growth Factor Relieves Inflammatory Signals in Staphylococcus aureus-Treated Human Epidermal Keratinocytes and Atopic Dermatitis-Like Skin Lesions in Nc/Nga Mice

Figure 1

Recombinant human epidermal growth factor (rhEGF) downregulated IL-6 expression induced by heat-killed S. aureus (HKSA). (a) 10 ng/mL rhEGF did not induce IL-6 or TSLP mRNA expression. However, HKSA with a multiplicity of infection (MOI) of 10 (10 MOI HKSA) increased the expression levels of IL-6 and TSLP mRNA; such expression was inhibited by 10 ng/mL of rhEGF. (b) As with the gene results, 10 ng/mL of rhEGF did not affect IL-6 or TSLP protein expression. 10 MOI HKSA induced the expression of IL-6 and TSLP protein; such expression was downregulated by 10 ng/mL of rhEGF. The results are presented as the mean ± STDEV, representative of three separate experiments. Asterisks indicate statistically significant differences ( < 0.05, < 0.01, and < 0.001).

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